Antioxidant activity of the organotellurium compound 3- 4-(N,N-dimethylamino)benzenetellurenyl propanesulfonic acid against oxidative stress in synaptosomal membrane systems and neuronal cultures.
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Abstract | :
Antioxidant activities of 3-[4-(N,N-dimethylamino) benzenetellurenyl]propanesulfonic acid sodium salt (NDBT) were evaluated in solution, red blood cells, synaptosomal membranes, and cultured hippocampal neuronal cells after exposure to peroxynitrite (ONOO(-)) and hydroxyl radicals. The organotellurium compound NDBT possesses significant activity towards hydrogen peroxide and/or the hydroxyl radical in solution, demonstrated by inhibition of hydroxylation of terephthalic acid. In addition, the compound displayed great antioxidant abilities as shown by: reduction of ONOO(-)-induced 2,7-dichlorofluorescein (DCF) fluorescence in synaptosomes; complete prevention of lipid peroxidation in synaptosomes caused by OH radicals (TBARS), and significant prevention of protein oxidation caused by ONOO(-) and OH, indexed by the levels of protein carbonyls in synaptosomes and neuronal cells. The presence of the compound abolished neuronal cell death caused by ONOO(-). Further, the compound was effective in preventing the oxidative changes in synaptosomal membrane protein conformation and crosslinking (EPR spin labeling). Finally, the organotellurium molecule attenuated peroxynitrite-induced, luminol-dependent chemiluminescence in red blood cells--an index of cellular oxidation. These findings demonstrate the great potential of the antioxidant and are consistent with the notion that NDBT may have a role to play in modulating oxidative stress in neurodegenerative disorders, including Alzheimer's disease. |
Year of Publication | :
2001
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Journal | :
Brain research
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Volume | :
911
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Issue | :
1
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Number of Pages | :
12-21
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Date Published | :
2001
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ISSN Number | :
0006-8993
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URL | :
https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(01)02541-0
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DOI | :
10.1016/s0006-8993(01)02541-0
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Short Title | :
Brain Res
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